2023-08-15 Chromeleon task

 

Document every step with screenshots and comments and prepare report!

  1. Install environment

Chromeleon Installation and SDK (Install first Chromeleon-Full-7.3.0.60919.iso) http://share.yellowrobot.xyz/1646159287-chromeleon

License code CMEI111115-PXHB-BRGC-G34C-2KXR-5YCJ-XCU6-3D1G-PJCA-LMMA-KRF0-LKXN-AGW8-6X0K-RLUT-YWUG-YFBN-J4T9-NWSP-V5F5-J0J

  1. To run simulator first lunch Instrument Service Manager image-20230815081541316

  2. Then run Instrument Configuration software. Add Instrument, then modules. Use primary Vanquish type of Modules. For Solvent Valves - Valco

image-20230815081624982 image-20230815081818990

  1. Set all modules to simulation mode in Module Settings. Initially add Quatanary pump, AutoSampler, ColumnCompartment and DAD

  2. Test connecting and controlling modules from Chromeleon (difference from Agilent ICF is that with this software you have all data processing available as well) image-20230815082050634

  3. Prepare instrument method and Injection inside Chromeleon. Use Instrument Method Wizard. (https://www.youtube.com/watch?app=desktop&v=RReWg73hmmg) image-20230815082328885

  4. Document carefully “Script Editor” when opening ready Instrument Method this is how GUI is translated into commands that also ChomSword sends to instrument image-20230815082546506

  5. Then Prepare Processing Method to do integration and find peaks https://www.youtube.com/watch?app=desktop&v=ZZOP9xAebU0 https://www.youtube.com/watch?v=ZZOP9xAebU0&pp=ygUhY2hyb21lbGVvbiBkYXRhIHByb2Nlc3NpbmcgbWV0aG9k

  6. Execute injection sequence with 2 runs, Injections contain Instrument method and Processing method. You add multiple injections to Queue and then execute it. Later verify results in Data section of Chormeleon https://www.youtube.com/watch?v=SwYuZXaDYLs&pp=ygUUY2hyb21lbGVvbiBpbmplY3Rpb24%3D image-20230815082947342

  7. Then repeat same procedure using ChromSword InstrumentThermo**, but this time we do not use Processing Method, but instead integrate using chromsword itself. Document and look carefully in Audit trail (it shows all actions that are sent to instrument). Results of chromatograms will be stored both in CSA RTPX and in Chromeleon. Notice that it will take up to 1min for data to show up in CSA, but it will be visible in Chromeleon immediately, because we read data from Chromeleon data vault which is not updated in real-time. image-20230815082159934

  8. Create more instrument configurations and test acquiring 2 runs using ChromSword

    1. Binary pump with 3 channels A1, A2, A3, B1, B2, B3; Sampler; Column Comp With Switching valves; DAD

    2. Quaternary pump, 2x Solvent valves, Sampler, Column Comp with column valves, DAD and VWD together

  9. Try to import data from Chromeleon in ReportViewer (this is useful if runs are acquired not using ChromSword, but just Chromeleon)